Introduction: Recent data from the German national resistance monitoring program GERM-Vet suggest that macrolide resistance (MR) is increasingly observed among Pasteurella multocida from cattle with respiratory infections in Germany. Macrolides are not only relevant antimicrobial agents in veterinary medicine, but are also considered critically important for humans. Here, we investigated the distribution of MR mechanisms present among bovine P. multocida from Germany. The isolates’ genetic relatedness was studied to better understand how MR might spread among these bacteria. Methods: In total, 166 P. multocida from cattle with respiratory infections included in GERM-Vet were investigated. Their genomes were sequenced to detect MR-mediating genes and mutations. The results were subsequently combined with metadata such as region and cattle density, to explore potential relations of certain MR mechanisms. Clustering techniques were applied to the sequence data to explore genetic relationships between the bacteria, followed by distance visualization in a heatmap and a minimum spanning tree. Results: The most common MR mechanism detected was the 23S rRNA mutation (n = 81), mediating a macrolide target site alteration. The genes mef(C) (macrolide efflux transporter) and mph(G) (enzymatic macrolide inactivation) were found in 71 isolates, always occurring in tandem and occasionally multiple times in the same isolate, while erm(T) (macrolide target site alteration) was detected less frequently (n = 5). The geospatial distribution showed regional clusters that coincided with areas of high cattle density. Genomic population analysis revealed six distinct clusters, of which in particular clusters 1, 3, 4, and 5 showed high internal similarity (median intra-cluster distance = 5 - 9 alleles), while cluster 6 was genetically most diverse (median intra-cluster distance = 31 alleles) and divergent from other clusters (median distance between clusters: 148 - 161 alleles). Conclusion: Several MR mechanisms were observed among bovine P. multocida from Germany, which differ from those commonly detected in other regions, such as North America. The determined genetic clusters highlight a substantial structure within the studied P. multocida population and provide valuable insights for downstream evolutionary analysis. However, geographic interpretations should be approached with caution due to the voluntary isolate provision within GERM-Vet and lacking antimicrobial usage data.